retinal ldv system model clbf 100 Search Results


non ionic detergent  (Thermo Fisher)
99
Thermo Fisher non ionic detergent
Non Ionic Detergent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd16b-pe  (Becton Dickinson)
90
Becton Dickinson cd16b-pe
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Cd16b Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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cell culture lysis buffer clb  (Promega)
90
Promega cell culture lysis buffer clb
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Cell Culture Lysis Buffer Clb, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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laser doppler velocimetry canon laser blood flowmeter clbf model 100  (Canon inc)
90
Canon inc laser doppler velocimetry canon laser blood flowmeter clbf model 100
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Laser Doppler Velocimetry Canon Laser Blood Flowmeter Clbf Model 100, supplied by Canon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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cell lysis buffer clb1  (Bayer AG)
90
Bayer AG cell lysis buffer clb1
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Cell Lysis Buffer Clb1, supplied by Bayer AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/retinal+ldv+system+model+clbf+100/cell+lysis+buffer+clb1/pmc05641147-163-14-18
Average 90 stars, based on 1 article reviews
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cd63 mouse igg1 clb clb gran 12 if  (Bio-Rad)
95
Bio-Rad cd63 mouse igg1 clb clb gran 12 if
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Cd63 Mouse Igg1 Clb Clb Gran 12 If, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/retinal+ldv+system+model+clbf+100/Rabbit+anti+Mouse+IgG1/pmc09335113__2021_280121_KAT_SUPPL-125-67-77
Average 95 stars, based on 1 article reviews
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streptavidin poly hrp  (MAST Group Ltd)
90
MAST Group Ltd streptavidin poly hrp
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Streptavidin Poly Hrp, supplied by MAST Group Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/retinal+ldv+system+model+clbf+100/streptavidin+biotinylated+horseradish+peroxide++hrp++complex/us08383351-383-5-8
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chaps lysis buffer  (Gold Biotechnology Inc)
94
Gold Biotechnology Inc chaps lysis buffer
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Chaps Lysis Buffer, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/retinal+ldv+system+model+clbf+100/CHAPS/pmc12557722__pnas%2E2517050122%2Esapp-16-10-35
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cpg-oligonucleotide dsp30  (TIB MOLBIOL)
90
TIB MOLBIOL cpg-oligonucleotide dsp30
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Cpg Oligonucleotide Dsp30, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/retinal+ldv+system+model+clbf+100/cpg+oligonucleotide+dsp30/pmc03386195-107-2-27
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cell lysis buffer clb  (GenDEPOT)
90
GenDEPOT cell lysis buffer clb
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Cell Lysis Buffer Clb, supplied by GenDEPOT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/retinal+ldv+system+model+clbf+100/ripa+buffer/10__1172_slash_jci156616-283-12-35
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laser blood flowmeter (clbf; model 100)  (Canon inc)
90
Canon inc laser blood flowmeter (clbf; model 100)
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Laser Blood Flowmeter (Clbf; Model 100), supplied by Canon inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/retinal+ldv+system+model+clbf+100/laser+blood+flowmeter/pm23701974-58-22-28
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laser blood flowmeter (clbf; model 100) - by Bioz Stars, 2026-09
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clenbuterol  (Dr Ehrenstorfer GmbH)
90
Dr Ehrenstorfer GmbH clenbuterol
A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + <t>CD16b</t> + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.
Clenbuterol, supplied by Dr Ehrenstorfer GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + CD16b + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Smad3 is essential for polarization of tumor-associated neutrophils in non-small cell lung carcinoma

doi: 10.1038/s41467-023-37515-8

Figure Lengend Snippet: A – C Confocal imaging and quantification show a marked accumulation of N2 TANs (CD206 + CD16b + cells) in NSCLC compared to adjacent normal lung tissue, which contrasts with the small population of N1 TANs (iNOS + CD16b + cells) (** P < 0.01 vs. normal lung; two-tailed t -test). D Three-dimensional confocal image showing SMAD3 activation (p-SMAD3, blue) in many N2 TANs (CD206 + CD16b + cells) in NSCLC. Correlation analysis shows that Smad3 activation ( E ) has a negative correlation with the proportion of N1 TANs ( F ) but does not correlate with the proportion of N2 TANs (Pearson correlation two-tailed test). G A low proportion of N1 TANs are significantly associated with higher patient mortality in NSCLC (log-rank (Mantel–Cox) test). Scale bar, 50 μm. A – D Data represents mean ± SEM of three patients/group. E – G Data based on a cohort of 72 patients. The exact P values of normal lung vs. NSCLC tissue are 1C . P = 0.0655(N1 TAN) and P = 0.004(N2 TAN). Source data are provided as a Source Data file.

Article Snippet: Monoclonal antibodies used for immunostaining and flow cytometry included; mCD206-PE (1:100, 141706, BioLegend, clone:C068C2), hCD206 (1:100, sc-376232, Santa Cruz,clone:C-10),hCD206-FITC(1:100, 321104, BioLegend, clone:15C2), iNOS (1:100, sc-7271, Santa Cruz,clone:C-11), TNF-α (1:100, sc-52746, Santa Cruz,clone:C-4), Icam1 (1:100, sc-8439, Santa Cruz,clone:G-5), p-Smad3 (paraffin section, 1:100, sc-517575, Santa Cruz,clone:1D9), CD16b-PE (1:100, 550868, BD Biosciences, clone:CLB-gran11.5), Ly6G-FITC (1:100, 127606, BioLegend,clone:1A8), Ly6G-PE (1:100, 127608, BioLegend,clone:1A8), Ly6G-APC (1:100, 127614, BioLegend,clone:1A8), p-Smad3 (1:200, 600-401-919, Rockland, Polyclonal), CD11b-APC (1:100, 553311, BD Biosciences,clone:M1/70 (RUO)).

Techniques: Imaging, Two Tailed Test, Activation Assay

A RT-PCR analysis showing that transfection of human PBDN with SMAD3 siRNA (S3KD-PBDN), but not with nonsense control siRNA (NC-PBDN), suppresses basal (control) and A549 conditioned medium (A549 CM) stimulated to increase in SMAD3 expression (** P < 0.01 vs. Control, ### P < 0.001 vs. NC-PBDN, n = 3 independent samples, one-way ANOVA). B Co-culture studies show enhanced cytotoxicity of S3KD-PBDN towards the human NSCLC cell line A549 (** P < 0.01, *** P < 0.001, n = 3 independent samples, two-tailed t -test). C , D NSG immunocompromised mice were inoculated with A549 cells and then received injections of human NC-PBDN or S3KD-PBDN on days 15 and 20 and then killed on day 25. C Adoptive transfer of S3KD-PBDN caused a significant reduction in tumor size and weight, which was not apparent with a transfer of NC-PBDN (*** P < 0.001 vs. untreated A549 tumors (Control), ### P < 0.001 vs. NC-PBDN, one-way ANOVA). D Immunostaining for human neutrophil markers showed that adoptive transfer of NC-PBDN resulted in a high proportion of TANs exhibiting an N2 phenotype (CD206 + CD16b + cells), whereas transfer of S3KD-PBDN resulted in a high proportion of TANs exhibiting an N1 phenotype (iNOS + CD16b + cells). Furthermore, p-SMAD3 staining was seen in a high proportion of N2, but not in N1 TANs. (* P < 0.05, ** P < 0.01, *** P < 0.001 vs. untreated A549 tumors (Control), ### P < 0.001 vs. NC-PBDN, one-way ANOVA) Scale bars, 50 μm. A , B Data represents mean ± SEM from three independent experiments. C , D Data represents mean ± SEM of 4 mice/group. The exact P values of NC-PBDN vs. S3KD-PBDN are 7A . P = 0.001 (Control), P = 0.0001(A549 CM-treated), P = 0.007 (NC-PBDN, Control vs. A549 CM-treated). 7B . P = 0.017 (1:2), P = 0.0157 (1:5), P = 0.0077 (1:10). Exact P values of PBDN treatment are 7C . P = 0.0002 (S3KD-PBDN vs. NC-PBDN, D20), P = 0.0002 (S3KD-PBDN vs Control, D20), P = 0.0001 (S3KD-PBDN vs. NC-PBDN, D25), P = 0.0001 (S3KD-PBDN vs. Control, D25), P = 0.0001 (S3KD-PBDN vs. NC-PBDN, tumor weight), P = 0.0001 (S3KD-PBDN vs. Control, tumor weight). 7D . P = 0.0001 (S3KD-PBDN vs. NC-PBDN, N1 TAN), P = 0.0001 (S3KD-PBDN vs. Control, N1 TAN), P = 0.0068 (NC-PBDN vs. Control, N1 TAN), P = 0.0001 (S3KD-PBDN vs. NC-PBDN, N2 TAN), P = 0.0404 (S3KD-PBDN vs. Control, N2 TAN), P = 0.0001 (NC-PBDN vs. Control, N2 TAN), P = 0.0001 (S3KD-PBDN vs. NC-PBDN, SMAD3 + N2 TAN), P = 0.0398 (S3KD-PBDN vs. Control, SMAD3 + N2 TAN), P = 0.0001 (NC-PBDN vs. Control, SMAD3 + N2 TAN). Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Smad3 is essential for polarization of tumor-associated neutrophils in non-small cell lung carcinoma

doi: 10.1038/s41467-023-37515-8

Figure Lengend Snippet: A RT-PCR analysis showing that transfection of human PBDN with SMAD3 siRNA (S3KD-PBDN), but not with nonsense control siRNA (NC-PBDN), suppresses basal (control) and A549 conditioned medium (A549 CM) stimulated to increase in SMAD3 expression (** P < 0.01 vs. Control, ### P < 0.001 vs. NC-PBDN, n = 3 independent samples, one-way ANOVA). B Co-culture studies show enhanced cytotoxicity of S3KD-PBDN towards the human NSCLC cell line A549 (** P < 0.01, *** P < 0.001, n = 3 independent samples, two-tailed t -test). C , D NSG immunocompromised mice were inoculated with A549 cells and then received injections of human NC-PBDN or S3KD-PBDN on days 15 and 20 and then killed on day 25. C Adoptive transfer of S3KD-PBDN caused a significant reduction in tumor size and weight, which was not apparent with a transfer of NC-PBDN (*** P < 0.001 vs. untreated A549 tumors (Control), ### P < 0.001 vs. NC-PBDN, one-way ANOVA). D Immunostaining for human neutrophil markers showed that adoptive transfer of NC-PBDN resulted in a high proportion of TANs exhibiting an N2 phenotype (CD206 + CD16b + cells), whereas transfer of S3KD-PBDN resulted in a high proportion of TANs exhibiting an N1 phenotype (iNOS + CD16b + cells). Furthermore, p-SMAD3 staining was seen in a high proportion of N2, but not in N1 TANs. (* P < 0.05, ** P < 0.01, *** P < 0.001 vs. untreated A549 tumors (Control), ### P < 0.001 vs. NC-PBDN, one-way ANOVA) Scale bars, 50 μm. A , B Data represents mean ± SEM from three independent experiments. C , D Data represents mean ± SEM of 4 mice/group. The exact P values of NC-PBDN vs. S3KD-PBDN are 7A . P = 0.001 (Control), P = 0.0001(A549 CM-treated), P = 0.007 (NC-PBDN, Control vs. A549 CM-treated). 7B . P = 0.017 (1:2), P = 0.0157 (1:5), P = 0.0077 (1:10). Exact P values of PBDN treatment are 7C . P = 0.0002 (S3KD-PBDN vs. NC-PBDN, D20), P = 0.0002 (S3KD-PBDN vs Control, D20), P = 0.0001 (S3KD-PBDN vs. NC-PBDN, D25), P = 0.0001 (S3KD-PBDN vs. Control, D25), P = 0.0001 (S3KD-PBDN vs. NC-PBDN, tumor weight), P = 0.0001 (S3KD-PBDN vs. Control, tumor weight). 7D . P = 0.0001 (S3KD-PBDN vs. NC-PBDN, N1 TAN), P = 0.0001 (S3KD-PBDN vs. Control, N1 TAN), P = 0.0068 (NC-PBDN vs. Control, N1 TAN), P = 0.0001 (S3KD-PBDN vs. NC-PBDN, N2 TAN), P = 0.0404 (S3KD-PBDN vs. Control, N2 TAN), P = 0.0001 (NC-PBDN vs. Control, N2 TAN), P = 0.0001 (S3KD-PBDN vs. NC-PBDN, SMAD3 + N2 TAN), P = 0.0398 (S3KD-PBDN vs. Control, SMAD3 + N2 TAN), P = 0.0001 (NC-PBDN vs. Control, SMAD3 + N2 TAN). Source data are provided as a Source Data file.

Article Snippet: Monoclonal antibodies used for immunostaining and flow cytometry included; mCD206-PE (1:100, 141706, BioLegend, clone:C068C2), hCD206 (1:100, sc-376232, Santa Cruz,clone:C-10),hCD206-FITC(1:100, 321104, BioLegend, clone:15C2), iNOS (1:100, sc-7271, Santa Cruz,clone:C-11), TNF-α (1:100, sc-52746, Santa Cruz,clone:C-4), Icam1 (1:100, sc-8439, Santa Cruz,clone:G-5), p-Smad3 (paraffin section, 1:100, sc-517575, Santa Cruz,clone:1D9), CD16b-PE (1:100, 550868, BD Biosciences, clone:CLB-gran11.5), Ly6G-FITC (1:100, 127606, BioLegend,clone:1A8), Ly6G-PE (1:100, 127608, BioLegend,clone:1A8), Ly6G-APC (1:100, 127614, BioLegend,clone:1A8), p-Smad3 (1:200, 600-401-919, Rockland, Polyclonal), CD11b-APC (1:100, 553311, BD Biosciences,clone:M1/70 (RUO)).

Techniques: Reverse Transcription Polymerase Chain Reaction, Transfection, Expressing, Co-Culture Assay, Two Tailed Test, Adoptive Transfer Assay, Immunostaining, Staining